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From the next day media was switched to neural differentiation medium and we kept feeding cells every 2 days

During the injection, use a dual-chamber syringe with 30 mM phosphate and 10 mM histamine

Fundamentals of Biochemistry

Multiple factors may influence the high redox potential of non-native disulfide bonds, including: 1) conformational strain induced by the formation of a non-native bond (Zhou et al., 1993), 2) low effective concentration of reacting thiol groups and 3) unfavorable electrostatic factors that affect the pK a of the corresponding thiols

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